Test ID: ADNA DNA Double-Stranded Antibodies, IgG, Serum
Reporting Name
DNA Double-Stranded Ab, IgG, SUseful For
Evaluating patients with signs and symptoms consistent with systemic lupus erythematosus (SLE)
Monitoring patients with documented SLE for flares in disease activity
Clinical Information
Double-stranded (ds, native) DNA (dsDNA) antibodies of the IgG class are an accepted criterion (American College of Rheumatology) for the diagnosis of systemic lupus erythematosus (SLE).(1-3) dsDNA antibodies are detectable in approximately 85% of patients with untreated SLE, and are rarely detectable in other connective tissue diseases. Weakly positive results caused by low-avidity antibodies to dsDNA are not specific for SLE and can occur in a variety of diseases.
Testing for IgG antibodies to dsDNA is indicated in patients who have a positive test for antinuclear antibodies (ANA) along with signs and symptoms that are compatible with the diagnosis of SLE. If the ANA test is negative, there is no reason to test for antibodies to dsDNA.(2)
The levels of IgG antibodies to dsDNA in serum are known to fluctuate with disease activity in lupus erythematosus, often increasing prior to an increase in inflammation and decreasing in response to therapy.(1,2)
Interpretation
A positive test result for double-stranded DNA (dsDNA) antibodies is consistent with the diagnosis of systemic lupus erythematosus.
A reference range study conducted at the Mayo Clinic demonstrated that, within a cohort of healthy adults (n=120), no individuals between the ages of 18 and 60 (n=78) had detectable anti-dsDNA antibodies. Above the age of 60 (n=42), 11.9% of individuals (n=5) had a borderline result for dsDNA antibodies and 4.8% of individuals (n=2) had a positive result.
Analytic Time
1 dayDay(s) and Time(s) Performed
Monday through Saturday; 4 p.m.
Clinical Reference
1. Tran T, Pisetsky D: Chapter 115. Detection of anti-DNA antibodies. In Manual of Molecular and Clinical Laboratory Immunology. Seventh edition. Edited by B Detrick, R Hamilton, JD Folds. Washington, DC, ASM Press, 2006, pp 1027-1032
2. Kavanaugh A, Tomar R, Reveille J, et al: Guidelines for use of the antinuclear antibody test and tests for specific autoantibodies to nuclear antigens. Arch Pathol Lab Med 2000;124:71-81
3. Tan EM, Cohen AS, Fries JF, et al: The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum 1982;25:1271-1277
Method Name
Enzyme-Linked Immunosorbent Assay (ELISA)
Specimen Type
SerumSpecimen Required
Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Specimen Volume: 0.5 mL
Specimen Minimum Volume
0.35 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Serum | Refrigerated (preferred) | 21 days | |
Frozen | 21 days |
Reference Values
<30.0 IU/mL (negative)
30.0-75.0 IU/mL (borderline)
>75.0 IU/mL (positive)
Negative is considered normal.
Reference values apply to all ages.
Test Classification
This test has been cleared, approved or is exempt by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
86225
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
ADNA | DNA Double-Stranded Ab, IgG, S | 33799-8 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
ADNA | DNA Double-Stranded Ab, IgG, S | 33799-8 |
Forms
If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.
mml-Behavioral, mml-Cerebrovascular, mml-Epilepsy, mml-Headache, mml-Movement-Disorders, mml-Demyelinating-Diseases, mml-Neuroimmunology, mml-Neuromuscular, mml-Autonomic, mml-Pediatric, mml-Spinal-Cord