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Test ID: BRUCB Brucella Culture, Blood

Reporting Name

Brucella Culture, B

Useful For

Diagnosis of brucellosis in blood specimens

Clinical Information

Brucella species are facultative intracellular Gram-negative-staining bacilli capable of producing the disease "brucellosis" in humans. Human disease likely is acquired by contact with animals infected with the organism (Brucella abortus, B suis, B melitensis, and occasionally B canis) either by direct contact or by ingestion of meat or milk. The signs and symptoms associated with brucellosis may include fever, night sweats, chills, weakness, malaise, headache, and anorexia. The physical examination may reveal lymphadenopathy and hepatosplenomegaly. A definitive diagnosis of brucellosis is made by recovering the organism from blood, fluid (including urine), or tissue specimens.

Interpretation

Isolation of a Brucella species indicates infection

 

Cultures of blood and/or bone marrow are positive in 70% to 90% of acute Brucella infections, but much less so in subacute or chronic infections. In these latter instances, culture yield is highest from the specific tissue involved, or serology may be necessary to establish diagnosis.

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
RMALD Ident by MALDI-TOF mass spec No, (Bill Only) No
ISAE Aerobe Ident by Sequencing No, (Bill Only) No

Testing Algorithm

When this test is ordered, the reflex tests may be performed and charged.

Analytic Time

14 days

Day(s) and Time(s) Performed

Monday through Sunday

Clinical Reference

1. Mandell GL, Bennett JE, Dolin R: Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases. Fourth edition. New York, Churchill Livingstone, 1995, pp 2053-2060

2. Procop GW, Church DL, Hall GS, et al: Chapter 9: Miscellaneous Fastidious Gram-Negative Bacilli. In Koneman's Color Atlas and Textbook of Diagnostic Microbiology. Seventh edition. Philadelphia, Wolters Kluwer, 2017, pp 472-595

Method Name

Conventional Culture Technique

Specimen Type

Whole Blood Culture


Advisory Information


Mayo Clinic Laboratories does not perform species identification of Brucella; these isolates should be submitted to the State Health Department laboratory.



Necessary Information


Specimen source is required.



Specimen Required


Container/Tube: Blood Isolator tube

Specimen Volume: Entire collection


Specimen Stability Information

Specimen Type Temperature Time
Whole Blood Culture Ambient 24 hours

Reference Values

No growth after 14 days

Test Classification

This test has been cleared, approved or is exempt by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

87081-Brucella Culture, Blood

87153-Aerobic Ident by Sequencing (if appropriate)

87077-Ident by MALDI-TOF mass spec (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
BRUCB Brucella Culture, B 551-2

 

Result ID Test Result Name Result LOINC Value
BRUCB Brucella Culture, B 551-2
Mayo Clinic Laboratories | Neurology Catalog Additional Information:

mml-CNS-Infections, mml-Pediatric