Test ID: ENAE Antibody to Extractable Nuclear Antigen Evaluation, Serum
Reporting Name
Ab to Extractable Nuclear Ag Eval,SUseful For
Evaluating patients with signs and symptoms of a connective tissue disease in whom the test for antinuclear antibodies is positive
Testing is not useful in patients without demonstrable antinuclear antibodies.
Clinical Information
SSA, SSB, SM, and RNP are extractable nuclear antigens (ENA) that occur in patients with several different connective tissue diseases. Scl 70 (topoisomerase 1) is a 100-kD nuclear and nucleolar enzyme. Scl 70 antibodies are considered to be specific for scleroderma (systemic sclerosis) and are found in up to 60% of patients with this connective tissue disease. Scl 70 antibodies are more common in patients with extensive cutaneous involvement and interstitial pulmonary fibrosis, and are considered a poor prognostic sign.(1) JO1 is a member of the amino acyl-tRNA synthetase family of enzymes found in all nucleated cells and a marker for the disease polymyositis.
For more information, see individual unit codes.
Interpretation
A positive result is consistent with a connective tissue disease.
For more information, see individual unit codes.
Profile Information
Test ID | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
SSA | SS-A/Ro Ab, IgG, S | Yes | Yes |
SSB | SS-B/La Ab, IgG, S | Yes | Yes |
SM | Sm Ab, IgG, S | Yes | Yes |
RNP | RNP Ab, IgG, S | Yes | Yes |
SCL70 | Scl 70 Ab, IgG, S | Yes | Yes |
JO1 | Jo 1 Ab, IgG, S | Yes | Yes |
Testing Algorithm
See Connective Tissue Disease Cascade (CTDC) in Special Instructions.
Report Available
Same day/1 to 2 daysDay(s) Performed
Monday through Saturday
Clinical Reference
1. Homburger H, Larsen S: Detection of specific antibodies. In Clinical Immunology: Principles and Practice. First edition. Edited by R Rich, T Fleisher, B Schwartz, et al. St. Louis, Mosby-Year Book, Inc, 1996, pp 2096-2109
2. Targoff I: Autoantibodies in polymyositis. Rheum Dis Clin North Am 1992;18:455
Method Name
Multiplex Flow Immunoassay
Specimen Type
SerumSpecimen Required
Container/Tube:
Preferred: Red top
Acceptable: Serum gel
Specimen Volume: 0.5 mL
Specimen Minimum Volume
0.35 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Serum | Refrigerated (preferred) | 21 days | |
Frozen | 21 days |
Special Instructions
Reference Values
SS-A/Ro ANTIBODIES, IgG
<1.0 U (negative)
≥1.0 U (positive)
Reference values apply to all ages.
SS-B/La ANTIBODIES, IgG
<1.0 U (negative)
≥1.0 U (positive)
Reference values apply to all ages.
Sm ANTIBODIES, IgG
<1.0 U (negative)
≥1.0 U (positive)
Reference values apply to all ages.
RNP ANTIBODIES, IgG
<1.0 U (negative)
≥1.0 U (positive)
Reference values apply to all ages.
Scl 70 ANTIBODIES, IgG
<1.0 U (negative)
≥1.0 U (positive)
Reference values apply to all ages.
Jo 1 ANTIBODIES, IgG
<1.0 U (negative)
≥1.0 U (positive)
Reference values apply to all ages.
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
86235 x 6
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
ENAE | Ab to Extractable Nuclear Ag Eval,S | 90228-8 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
JO1 | Jo 1 Ab, IgG, S | 33571-1 |
RNP | RNP Ab, IgG, S | 29958-6 |
SCL70 | Scl 70 Ab, IgG, S | 47322-3 |
SM | Sm Ab, IgG, S | 18323-6 |
SSA | SS-A/Ro Ab, IgG, S | 33610-7 |
SSB | SS-B/La Ab, IgG, S | 33613-1 |
Forms
If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.
mml-Behavioral, mml-Cerebrovascular, mml-Epilepsy, mml-Headache, mml-Movement-Disorders, mml-Demyelinating-Diseases, mml-Neuroimmunology, mml-Neuromuscular, mml-Autonomic, mml-Pediatric, mml-Spinal-Cord, mml-Neuro-ophthalmology