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Test ID: FIBR Fibroblast Culture for Biochemical and Molecular Testing, Tissue

Reporting Name

Fibroblast Culture

Useful For

Obtaining cultured cells for specialized testing including enzymatic and molecular genetic

Clinical Information

Cultures of skin fibroblasts are useful for specialized tests requiring skin cells. These cells can be cultured and tested at Mayo Clinic Laboratories or sent to external laboratories performing these specialized tests. In addition, cells are frozen for a minimum of 3 years for potential future studies on cultured cells or for molecular genetic testing.

Testing Algorithm

This processing test is for culturing skin fibroblasts or other biopsies for biochemical or molecular genetic studies. No analysis or interpretation of results is performed.

 

When this test is ordered, cryopreservation will always be performed at an additional charge. However, for multiple assays on a patient utilizing the ordered fibroblast culture, only one culture is required regardless of the number of assays ordered.

 

Once confluent flasks are established, the cultures are sent to other laboratories, either within Mayo Clinic Laboratories or to external sites, based on the specific testing requested. To avoid delays, indicate testing to be performed and provide external lab paperwork for send out testing.

 

If viable cells are not obtained within 30 days, client will be notified.

 

Mycoplasma screening will be performed on all successfully grown fibroblast cultures. If Mycoplasma is detected, all pending fibroblast clinical testing will be cancelled, and the samples will be discarded.

Report Available

40 to 45 days

Day(s) Performed

Varies

Method Name

FIBR: Cultivated from Biopsy as Monolayer

CRYOB: Fibroblast Subculture followed by Cryopreservation and Storage

Specimen Type

Tissue


Ordering Guidance


This test is most useful for skin biopsy specimens. The specimen is cryopreserved for a minimum of 3 years.

 

This test is not appropriate for products of conception (POC) or chorionic villus sampling (CVS) specimens. If this test is ordered for either a POC or CVS specimen, the test will be canceled and CULFB / Fibroblast Culture for Molecular Testing, Chorionic Villi/Products of Conception will be performed as the appropriate test.

 

If ordering this test to obtain DNA for testing at a different laboratory location, see Necessary Information.



Necessary Information


1. Include patient clinical history with request. This information will be used for any testing performed on the cultured cells.

2. Indicate the tests to be performed on the fibroblast culture cells.

Note: For courtesy DNA extraction from fibroblasts for testing performed at locations other than Mayo Clinic Laboratories (MCL), a MCL Test ID and test requisition for the requested laboratory are required. Prior to specimen collection, call Mayo Lab Inquiry at 800-533-1710 to confirm availability of send-out testing options as courtesy DNA extraction will not be performed for tests not in the MCL Referral catalog.



Specimen Required


Submit only 1 of the following specimens:

 

Specimen Type: Cultured fibroblasts

Container/Tube: T-75 or T-25 flask

Specimen Volume: 1 Full T-75 flask or 2 full T-25 flasks

Specimen Stability Information: Ambient/Refrigerated

 

Specimen Type: Skin biopsy

Supplies: Fibroblast Biopsy Transport Media (T115)

Container/Tube: Sterile container with any standard cell culture media (eg, minimal essential media, RPMI 1640). The solution should be supplemented with 1% penicillin and streptomycin. Tubes of culture media can be supplied upon request (Eagle's minimum essential medium with 1% penicillin and streptomycin).

Specimen Volume: 4-mm punch

Specimen Stability Information: Refrigerated/Ambient


Specimen Stability Information

Specimen Type Temperature Time Special Container
Tissue Varies

Reference Values

Not applicable

Test Classification

Not Applicable

CPT Code Information

88233-Fibroblast culture

88240-Cryopreservation for biochemical studies

LOINC Code Information

Test ID Test Order Name Order LOINC Value
FIBR Fibroblast Culture 96300-9

 

Result ID Test Result Name Result LOINC Value
8482 Fibroblast Culture 96300-9

Additional Tests

Test ID Reporting Name Available Separately Always Performed
CRYOB Cryopreserve for Biochem Studies No Yes
Mayo Clinic Laboratories | Neurology Catalog Additional Information:

mml-Behavioral, mml-Epilepsy, mml-Movement-Disorders, mml-Demyelinating-Diseases, mml-Neuromuscular, mml-Autonomic, mml-Pediatric, mml-Spinal-Cord, mml-Neurometabolic, mml-Neuro-ophthalmology