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Test ID: FIBR Fibroblast Culture, Tissue

Reporting Name

Fibroblast Culture

Useful For

Obtaining cultured cells for specialized testing including enzymatic and molecular genetic

Clinical Information

Cultures of skin fibroblasts are useful for specialized tests requiring skin cells. These cells can be cultured and tested at Mayo Clinic or sent to external laboratories  performing these specialized tests. In addition, cells are frozen for at least 3 years for potential future studies on cultured cells or for molecular genetic testing.

Testing Algorithm

This processing test is for culturing skin fibroblasts or other biopsy for biochemical or molecular genetic studies. No analysis or interpretation of results is performed.

                                                                

When this test is ordered, cryopreservation will always be performed at an additional charge. However, for multiple assays on a patient utilizing the ordered fibroblast culture, only 1 culture is required regardless of the number of assays ordered.

 

Once confluent flasks are established, the cultures are sent to other laboratories, either within Mayo Clinic or to external sites, based on the specific testing requested.

If viable cells are not obtained within 30 days, client will be notified.

 

Mycoplasma screening will be performed on all successfully grown fibroblast cultures. If Mycoplasma is detected, all pending fibroblast clinical testing will be cancelled and the samples will be discarded.

Analytic Time

40-45 days depending on rapidity of growth

Day(s) and Time(s) Performed

Varies

Method Name

FIBR: Cultivated from Biopsy as Monolayer

CRYOB: Fibroblast Subculture Followed by Cryopreservation and Storage

Specimen Type

Tissue


Advisory Information


This test should be ordered for skin or other biopsy specimens.

 

If the specimen is a product of conception or chorionic villi, order CULFB / Fibroblast Culture for Genetic Testing, Tissue.



Necessary Information


1. Include patient clinical history with request. This information will be used for any testing performed on the cultured cells.

2. Indicate the tests to be performed on the fibroblast culture cells. If send out testing is requested, the external lab paperwork must be submitted.



Specimen Required


Submit only 1 of the following specimens:

 

Specimen Type: Cultured fibroblasts

Container/Tube: T-75 or T-25 flask

Specimen Volume: 1 Full T-75 flask or 2 full T-25 flasks

Specimen Stability Information: Ambient/Refrigerated

 

Specimen Type: Skin biopsy

Supplies: Fibroblast Biopsy Transport Media (T115)

Container/Tube: Sterile container with any standard cell culture media (eg, minimal essential media, RPMI 1640). The solution should be supplemented with 1% penicillin and streptomycin. Tubes of culture media can be supplied upon request (Eagle's minimum essential medium with 1% penicillin and streptomycin).

Specimen Volume: 4-mm punch

Specimen Stability Information: Refrigerated/Ambient


Specimen Stability Information

Specimen Type Temperature Time Special Container
Tissue Varies

Reference Values

Not applicable

Test Classification

Not Applicable

CPT Code Information

88233-Fibroblast culture

88240-Cryopreservation for biochemical studies

LOINC Code Information

Test ID Test Order Name Order LOINC Value
FIBR Fibroblast Culture 96300-9

 

Result ID Test Result Name Result LOINC Value
8482 Fibroblast Culture 96300-9

Additional Tests

Test ID Reporting Name Available Separately Always Performed
CRYOB Cryopreserve for Biochem Studies No Yes

Forms

Biochemical Genetics Patient Information (T602) in Special Instructions.

Mayo Clinic Laboratories | Neurology Catalog Additional Information:

mml-Behavioral, mml-Epilepsy, mml-Movement-Disorders, mml-Demyelinating-Diseases, mml-Neuromuscular, mml-Autonomic, mml-Pediatric, mml-Spinal-Cord, mml-Neurometabolic, mml-Neuro-ophthalmology