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Test ID: JO1 Jo 1 Antibodies, IgG, Serum

Reporting Name

Jo 1 Ab, IgG, S

Useful For

Evaluating patients with signs and symptoms compatible with a connective tissue disease, especially those patients with muscle pain and limb weakness, concomitant pulmonary signs and symptoms, Raynaud phenomenon, and arthritis

 

Testing for antibodies to Jo 1 is not useful in patients with a negative test for antinuclear antibodies

Clinical Information

Jo 1 (histidyl tRNA synthetase) is a member of the amino acyl-tRNA synthetase family of enzymes found in all nucleated cells. Jo 1 antibodies in patients with polymyositis bind to conformational epitopes of the enzyme protein and inhibit its catalytic activity in vitro.(1)

 

Jo 1 antibodies are a marker for the disease polymyositis, and occur most commonly in myositis patients who also have interstitial lung disease. The antibodies occur in up to 50% of patients with interstitial pulmonary fibrosis and symmetrical polyarthritis.(2)

 

See Connective Tissue Disease Cascade (CTDC) in Special Instructions.

Interpretation

A positive result for Jo 1 antibodies is consistent with the diagnosis of polymyositis and suggests an increased risk of pulmonary involvement with fibrosis in such patients.

Testing Algorithm

See Connective Tissue Disease Cascade (CTDC) in Special Instructions.

Analytic Time

1 day

Day(s) and Time(s) Performed

Monday through Saturday; 4 p.m.

Clinical Reference

1. Targoff I: Autoantibodies in polymyositis. Rheum Dis Clin North Am 1992;18:455

2. Leff R, Sherman J, Plotz P: Chapter 65: Inflammatory muscle diseases. In Clinical Immunology Principles and Practice, Second edition. Edited by R Rich, T Fleisher, W Shearer, B Kotzin, et al. St. Louis, Mosby-Year Book, 2001, pp 65.1-65.8

Method Name

Multiplex Flow Immunoassay

Specimen Type

Serum


Specimen Required


Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 0.5 mL


Specimen Minimum Volume

0.35 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 21 days
  Frozen  21 days

Reference Values

<1.0 U (negative)

≥1.0 U (positive)

Reference values apply to all ages.

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86235

LOINC Code Information

Test ID Test Order Name Order LOINC Value
JO1 Jo 1 Ab, IgG, S 33571-1

 

Result ID Test Result Name Result LOINC Value
JO1 Jo 1 Ab, IgG, S 33571-1
Mayo Clinic Laboratories | Neurology Catalog Additional Information:

mml-Behavioral, mml-Demyelinating-Diseases, mml-Neuroimmunology, mml-Neuromuscular, mml-Pediatric, mml-Spinal-Cord