Test ID: JO1 Jo 1 Antibodies, IgG, Serum
Reporting Name
Jo 1 Ab, IgG, SUseful For
Evaluating patients with signs and symptoms compatible with a connective tissue disease, especially those patients with muscle pain and limb weakness, concomitant pulmonary signs and symptoms, Raynaud phenomenon, and arthritis
Testing for antibodies to Jo 1 is not useful in patients with a negative test for antinuclear antibodies
Clinical Information
Jo 1 (histidyl tRNA synthetase) is a member of the amino acyl-tRNA synthetase family of enzymes found in all nucleated cells. Jo 1 antibodies in patients with polymyositis bind to conformational epitopes of the enzyme protein and inhibit its catalytic activity in vitro.(1)
Jo 1 antibodies are a marker for the disease polymyositis, and occur most commonly in myositis patients who also have interstitial lung disease. The antibodies occur in up to 50% of patients with interstitial pulmonary fibrosis and symmetrical polyarthritis.(2)
See Connective Tissue Disease Cascade (CTDC) in Special Instructions.
Interpretation
A positive result for Jo 1 antibodies is consistent with the diagnosis of polymyositis and suggests an increased risk of pulmonary involvement with fibrosis in such patients.
Testing Algorithm
See Connective Tissue Disease Cascade (CTDC) in Special Instructions.
Report Available
1 to 3 daysDay(s) Performed
Monday through Saturday
Clinical Reference
1. Targoff I: Autoantibodies in polymyositis. Rheum Dis Clin North Am 1992;18:455
2. Leff R, Sherman J, Plotz P: Chapter 65: Inflammatory muscle diseases. In Clinical Immunology Principles and Practice, Second edition. Edited by R Rich, T Fleisher, W Shearer, B Kotzin, et al. St. Louis, Mosby-Year Book, 2001, pp 65.1-65.8
Method Name
Multiplex Flow Immunoassay
Specimen Type
SerumSpecimen Required
Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Specimen Volume: 0.5 mL
Specimen Minimum Volume
0.35 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Serum | Refrigerated (preferred) | 21 days | |
Frozen | 21 days |
Special Instructions
Reference Values
<1.0 U (negative)
≥1.0 U (positive)
Reference values apply to all ages.
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
86235
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
JO1 | Jo 1 Ab, IgG, S | 33571-1 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
JO1 | Jo 1 Ab, IgG, S | 33571-1 |
mml-Behavioral, mml-Demyelinating-Diseases, mml-Neuroimmunology, mml-Neuromuscular, mml-Pediatric, mml-Spinal-Cord