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HelpTest ID: LADV Adenovirus, Molecular Detection, PCR, Varies
Reporting Name
Adenovirus PCRUseful For
Aiding in the diagnosis of adenovirus infections
Clinical Information
Human adenoviruses cause a variety of diseases, including pneumonia, cystitis, conjunctivitis, diarrhea, hepatitis, myocarditis, and encephalitis. In humans, adenoviruses have been recovered from almost every organ system. Infections can occur at any time of the year and in all age groups. Currently, there are over 50 adenovirus serotypes that have been grouped into 6 separate subgenera.
Although adenovirus can be recovered in cell culture, it can take up to 3 weeks for the virus to be identified by culture methods (Mayo Clinic's shell vial culture provides more rapid results, reported at 2 and 5 days). Serological tests have faster turnaround times but can be less sensitive compared to culture. Polymerase chain reaction assays offer a rapid, specific, and sensitive means of diagnosis by detecting adenovirus DNA.
Interpretation
A positive result indicates the presence of adenovirus DNA in the clinical specimen.
A negative result does not rule out the presence of adenovirus because viral DNA may be present at levels below the detection limits of this assay.
Report Available
2 to 5 daysDay(s) Performed
Monday through Sunday
Clinical Reference
1. Florescu DF, Schaenman JM. Adenovirus in solid organ transplant recipients: Guidelines from the American Society of Transplantation Infectious Diseases Community of Practice. Clin Transplant. 2019;33(9):e13527
2. Buckwalter SP, Teo R, Espy MJ, Sloan LM, Smith TF, Pritt BS. Real-time qualitative PCR for 57 human adenovirus types from multiple specimen sources. J Clin Microbiol. 2012;50(3):766-771. doi:10.1128/jcm.05629-11
3. Ebner K, Pinsker W, Lion T. Comparative sequence analysis of the hexon gene in the entire spectrum of human adenovirus serotypes: phylogenetic, taxonomic, and clinical implications. J Virol. 2005;79(20):12635-12642
4. Ebner K, Suda M, Watzinger F, Lion T. Molecular detection and quantitative analysis of the entire spectrum of human adenoviruses by a two-reaction real-time PCR assay. J Clin Microbiol. 2005;43(7):3049-3053
5. Jothikumar N, Cromeans TL, Hill VR, Lu X, Sobsey MD, Erdman DD. Quantitative real-time PCR assays for the detection of human adenoviruses and identification of serotypes 40 and 41. Appl Environ Microbiol. 2005;71(6):3131-3136
6. Robinson C, Echavarria M: Adenovirus. In: Murray PR, Baron EJ, Jorgensen JH, eds. Manual of Clinical Microbiology. ASM Press; 2007:1589-1600
7. Kaneko H, Maruko I, Iida T, et al. The possibility of human adenovirus detection from the conjunctiva in asymptomatic cases during a nosocomial infection. Cornea. 2008;27(5):527-530
Method Name
Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization
Specimen Type
VariesNecessary Information
1. Specimen source is required.
2. Source information must include main anatomical site of collection.
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Body fluid
Sources: Pleural, peritoneal, ascites, pericardial, or amniotic
Container/Tube: Sterile container
Specimen Volume: 0.5 mL
Collection Instructions: Do not centrifuge.
Specimen Type: Respiratory
Sources: Bronchial washing, bronchoalveolar lavage, nasopharyngeal aspirate or washing, sputum, or tracheal aspirate
Container/Tube: Sterile container
Specimen Volume: 1 mL
Specimen Type: Spinal fluid
Container/Tube: Sterile vial
Specimen Volume: 0.5 mL
Collection Instructions: Do not centrifuge.
Specimen Type: Stool
Supplies: Stool Collection Kit, Random (T635)
Container/Tube: Sterile container
Specimen Volume: 1 g
Specimen Type: Swab
Sources: Nasal, throat, respiratory, genital, or ocular
Supplies:
-Culturette (BBL Culture Swab) (T092)
-BD E-Swab (T853)
-M4-RT (T605)
Container/Tube: Multimicrobe media (M4-RT, M4, M5, Bartels, or Jiangsu) and E-Swab or Culturette
Specimen Volume: Entire specimen
Collection Instructions: Place swab back into a multimicrobe media.
Specimen Type: Tissue
Supplies: M4-RT (T605)
Container/Tube: Sterile container containing 1 mL to 2 mL of sterile saline or multimicrobe medium (M4-RT, M4, M5, Bartels, or Jiangsu)
Specimen Volume: Entire collection
Collection Instructions: Collect fresh tissue specimen.
Specimen Type: Urine
Container/Tube: Sterile container
Specimen Volume: 1 mL
Collection Instructions: Collect a random urine specimen.
Specimen Minimum Volume
Body fluid and respiratory specimens: 0.5 mL
Spinal fluid and urine: 0.3 mL
Stool: 0.5 g
Swab or Tissue: See Specimen Required
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Refrigerated (preferred) | 7 days |
| Frozen | 7 days |
Reference Values
Negative
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
87798
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| LADV | Adenovirus PCR | 39528-5 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| SRC65 | Specimen Source | 31208-2 |
| 89074 | Adenovirus PCR | 39528-5 |
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.
mml-CNS-Infections, mml-Pediatric