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Test ID: LCMV Cytomegalovirus (CMV), Molecular Detection, PCR, Varies

Reporting Name

Cytomegalovirus PCR

Useful For

Rapid qualitative detection of cytomegalovirus (CMV) DNA

 

This test is not intended for the monitoring of cytomegalovirus (CMV) disease progression.

Clinical Information

Infection with cytomegalovirus (CMV) is a significant cause of morbidity and mortality in transplant recipients and other immunocompromised hosts. Specific neurologic syndromes associated with CMV infection include subacute radiculomyelopathy, peripheral neuropathy, and encephalitis. 

 

CMV-associated central nervous system (CNS) disease occurs most commonly in immunocompromised patients. Histologic evidence of CMV infections in autopsy brain tissue was identified in 20% to 40% of AIDS patients. In 2 separate studies, CMV (DNA) was the most common herpesvirus (29/181, 16/49) detected from cerebrospinal fluid of patients with AIDS. 

 

CNS infections with CMV can also occur in immunocompetent patients. CMV is a leading cause of congenital viral infections worldwide, and laboratory testing by real-time PCR is useful in the diagnosis of neonatal CMV disease.

Interpretation

Detection of cytomegalovirus (CMV) DNA in a specimen supports the clinical diagnosis of infection due to this virus.

 

Studies indicate that CMV DNA is not detected by PCR in cerebrospinal fluid from patients without central nervous system disease caused by this virus.

Analytic Time

Same day/1 day

Day(s) and Time(s) Performed

Monday through Saturday; 6 p.m.; Sunday: 1 p.m.

Clinical Reference

1. Espy M, Binnicker MJ: Comparison of six real-time PCR assays for the qualitative detection of cytomegalovirus in clinical specimens. J Clin Microbiol 2013:51(11):3749-3752

2. Petito CK, Cho ES, Lemann W, et al: Neuropathy of acquired immunodeficiency syndrome (AIDS): an autopsy review. J Neuropathol Exp Neurol 1986 November;45(6):635-646

3. Cinque P, Vago L, Dahl H, et al: Polymerase chain reaction on cerebrospinal fluid for diagnosis of virus-associated opportunistic diseases of the central nervous system in HIV-infected patients. AIDS 1996 August;10(9):951-958

4. Broccolo F, Iulioano R, Careddu AM, et al: Detection of lymphotropic herpesvirus DNA by polymerase chain reaction in cerebrospinal fluid of AIDS patients with neurological disease. Acta Virol 2000 June-August;44(3):137-143

5. Prosch S, Schielke E, Reip A, et al: Human cytomegalovirus (HCMV) encephalitis in an immunocompetent young person and diagnostic reliability of HCMV DNA PCR using cerebrospinal fluid of nonimmunosuppressed patients. J Clin Microbiol 1998 December;36(12):3636-3640

6. Sia IG, Patel R: New strategies for prevention and therapy of cytomegalovirus infection and disease in solid-organ transplant recipients. Clin Microbiol Rev 2000;13:83-121

Method Name

Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization

Specimen Type

Varies


Advisory Information


For plasma specimens order CMVQN / Cytomegalovirus (CMV) DNA Detection and Quantification by Real-Time PCR, Plasma.



Necessary Information


Specimen source is required.



Specimen Required


Submit only 1 of the following specimens:

 

Supplies: Aliquot Tube, 5 mL (T465)

Specimen Type: Fluid

Sources: Spinal, pleural, peritoneal, ascites, pericardial, amniotic, or ocular

Container/Tube:

Preferred: Sterile screw-cap 5-mL aliquot tube

Acceptable: Sterile Container

Specimen Volume: 0.5 mL

Collection Instructions: Do not centrifuge.

 

Supplies: Aliquot Tube, 5 mL (T465)

Specimen Type: Fluid

Sources: Respiratory; bronchial washing, bronchoalveolar lavage, nasopharyngeal aspirate or washing, sputum, or tracheal aspirate

Container/Tube:

Preferred: Sterile screw-cap 5-mL aliquot tube

Acceptable: Sterile container

Specimen Volume: 1.5 mL

 

Supplies:

Culturette (BBL Culture Swab) (T092)

M4-RT (T605)

Specimen Type: Swab

Sources: Genital; cervix, vagina, urethra, anal/rectal, or other genital sources

Container/Tube: multimicrobe media (M4-RT) (T605) and ESwabs

Collection Instructions: Place swab back into multimicrobe media (M4-RT, M4, or M5) 

 

Supplies:

Culturette (BBL Culture Swab) (T092)

M4-RT Media (T605)

Specimen Type: Swab

Sources: Miscellaneous; dermal, eye, nasal, saliva, or throat

Container/Tube: multimicrobe media (M4-RT) (T605) and ESwabs

Collection Instructions: Place swab back into multimicrobe media (M4-RT, M4, or M5)

 

Supplies: M4-RT (T605)

Specimen Type: Tissue

Sources: Brain, colon, kidney, liver, lung, etc.

Container/Tube: Sterile container containing 1 mL to 2 mL of sterile saline or multimicrobe medium (M4-RT [T605], M4, or M5)

Specimen Volume: Entire collection

Collection Instructions: Submit only fresh tissue in multimicrobe media (M4-RT) (T605) or a sterile container with 1 to 2 mL sterile saline

 

Specimen Type: Urine

Container/Tube: Sterile container

Specimen Volume: 1 mL

Collection Instructions: Collect a random urine specimen.

 

Specimen Type: Bone marrow

Container/Tube: Lavender top (EDTA)

Specimen Volume: 0.5 mL


Specimen Minimum Volume

Body Fluid, Ocular Fluid, Spinal Fluid, or Urine: 0.3 mL
Respiratory Specimens: 1 mL
Tissue: 2 × 2-mm biopsy

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) 7 days
  Frozen  7 days

Reference Values

Negative

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

87496

LOINC Code Information

Test ID Test Order Name Order LOINC Value
LCMV Cytomegalovirus PCR 5000-5

 

Result ID Test Result Name Result LOINC Value
SRC66 Specimen Source 31208-2
81240 Cytomegalovirus PCR 5000-5

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.

Mayo Clinic Laboratories | Neurology Catalog Additional Information:

mml-CNS-Infections, mml-Pediatric, mml-Spinal-Cord