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HelpTest ID: LEBV Epstein-Barr Virus (EBV), Molecular Detection, PCR, Varies
Reporting Name
Epstein-Barr Virus PCRUseful For
Rapid qualitative detection of Epstein-Barr virus (EBV) DNA in specimens for laboratory diagnosis of disease due to this virus
Clinical Information
Epstein-Barr virus (EBV) is the causative agent of infectious mononucleosis, Burkitt lymphoma, and in Southern China, nasopharyngeal carcinoma. EBV-associated central nervous system (CNS) disease is most commonly associated with primary CNS lymphoma in patients with AIDS. In addition, CNS infection associated with the detection of EBV DNA can be seen in immunocompetent patients.
Interpretation
Detection of Epstein-Barr virus (EBV) DNA in cerebrospinal fluid (CSF) supports the clinical diagnosis of central nervous system (CNS) disease due to the virus. EBV DNA is not detected in CSF from patients without CNS disease caused by this virus.
Analytic Time
Monday through Friday: 2 days; Saturday, Sunday: 3 daysDay(s) and Time(s) Performed
Monday through Friday; 6 a.m.
Clinical Reference
1. Tachikawa N, Goto M, Hoshino Y, et al: Detection of Toxoplasma gondii, Epstein-Barr virus, and JC virus DNAs in the cerebrospinal fluid in acquired immunodeficiency syndrome patients with focal central nervous system complications. Intern Med 1999;38(7):556-562
2. Antinori A, Cingolani A, De Luca A, et al: Epstein-Barr virus in monitoring the response to therapy of acquired immunodeficiency syndrome-related primary central nervous system lymphoma. Ann Neurol 1999;45(2):259-261
3. Cingolani A, De Luca A, Larocca LM, et al: Minimally invasive diagnosis of acquired immunodeficiency syndrome-related primary central nervous system lymphoma. J Natl Cancer Inst 1998;90(8):364-369
4. Niller HH, Wolf H, Minarovits J: Regulation and dysregulation of Epstein-Barr virus latency: implications for the development of autoimmune disease. Autoimmunity 2008:41(4):298-328
5. Studahl M, Hagberg L, Rekvdar E, Bergstrom T: Herpesvirus DNA detection in cerebrospinal fluid: difference in clinical presentation between alph-, beta-, and gamma-herpes viruses. Scand J Infect Dis 2000;32(3):237-248
6. Lau AH, Soltys K, Sindhi RK, et al: Chronic high Epstein-Barr viral load carriage in pediatric small bowel transplant recipients. Pediatr Transplant 2010;14(4):549-553 Epub: Jan 20
Method Name
Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization
Specimen Type
VariesNecessary Information
Specimen source is required.
Specimen Required
Supplies: Aliquot Tube, 5 mL (T465)
Specimen Type: Fluid
Sources: Spinal fluid, sterile body fluids (peritoneal fluid/ascites, pericardial fluid, pleural fluid/thoracentesis, amniotic, or ocular
Preferred: Sterile screw-cap 5-mL aliquot tube
Acceptable: Sterile container
Specimen Volume: 0.5 mL
Collection Instructions: Do not centrifuge.
Supplies: Aliquot Tube, 5 mL (T465)
Specimen Type: Fluid
Sources: Respiratory; bronchial washing, bronchoalveolar lavage, nasopharyngeal aspirate or washing, sputum, or tracheal aspirate
Container/Tube:
Preferred: Sterile screw-cap 5-mL aliquot tube
Acceptable: Sterile container
Specimen Volume: 1.5 mL
Supplies:
-Culturette (BBL Culture Swab) (T092)
-M4-RT (T605)
-Bartels FlexTrans VTM-3 mL (T892)
-Jiangsu VTM-3 mL (T891)
Specimen Type: Swab
Sources: Eye swabs and upper respiratory swabs (nasal, throat)
Container/Tube: Multimicrobe media (M4-RT) and Eswabs
Collection Instructions: Place swab back into multimicrobe media (M4-RT, M4 or M5)
Specimen Type: Bone marrow
Container/Tube: Lavender top (EDTA) only
Specimen Volume: 0.5 mL
Additional Information: Clotted specimens will be rejected.
Supplies:
-M4-RT (T605)
-Bartels FlexTrans VTM-3 mL (T892)
-Jiangsu VTM-3 mL (T891)
Specimen Type: Tissue
Sources: Brain, colon, kidney, liver, lung, etc.
Preferred: Multimicrobe medium (M4-RT)
Acceptable: Sterile container containing 1 mL to 2 mL of sterile saline or multimicrobe medium (M4-RT, M4 or M5)
Specimen Volume: Entire collection
Collection Instructions: Submit only fresh tissue.
Specimen Minimum Volume
Body Fluid, Ocular Fluid, Spinal Fluid: 0.3 mL
Respiratory Specimens: 1 mL
Tissue: 2 × 2-mm biopsy
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Refrigerated (preferred) | 7 days | |
| Frozen | 7 days |
Reference Values
Negative
Test Classification
This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.CPT Code Information
87798
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| LEBV | Epstein-Barr Virus PCR | 23858-4 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| SRC67 | Specimen Source | 31208-2 |
| 81239 | Epstein-Barr Virus PCR | 23858-4 |
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.
mml-CNS-Infections, mml-Pediatric, mml-Spinal-Corde