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Test ID: LEBV Epstein-Barr Virus (EBV), Molecular Detection, PCR, Varies

Reporting Name

Epstein-Barr Virus PCR

Useful For

Rapid qualitative detection of Epstein-Barr virus (EBV) DNA in specimens for laboratory diagnosis of disease due to this virus

Clinical Information

Epstein-Barr virus (EBV) is the causative agent of infectious mononucleosis, Burkitt lymphoma, and in Southern China, nasopharyngeal carcinoma. EBV-associated central nervous system (CNS) disease is most commonly associated with primary CNS lymphoma in patients with AIDS. In addition, CNS infection associated with the detection of EBV DNA can be seen in immunocompetent patients.

Interpretation

Detection of Epstein-Barr virus (EBV) DNA in cerebrospinal fluid (CSF) supports the clinical diagnosis of central nervous system (CNS) disease due to the virus. EBV DNA is not detected in CSF from patients without CNS disease caused by this virus.

Analytic Time

Monday through Friday: 2 days; Saturday, Sunday: 3 days

Day(s) and Time(s) Performed

Monday through Friday; 6 a.m.

Clinical Reference

1. Tachikawa N, Goto M, Hoshino Y, et al: Detection of Toxoplasma gondii, Epstein-Barr virus, and JC virus DNAs in the cerebrospinal fluid in acquired immunodeficiency syndrome patients with focal central nervous system complications. Intern Med 1999;38(7):556-562 

2. Antinori A, Cingolani A, De Luca A, et al: Epstein-Barr virus in monitoring the response to therapy of acquired immunodeficiency syndrome-related primary central nervous system lymphoma. Ann Neurol 1999;45(2):259-261

3. Cingolani A, De Luca A, Larocca LM, et al: Minimally invasive diagnosis of acquired immunodeficiency syndrome-related primary central nervous system lymphoma. J Natl Cancer Inst 1998;90(8):364-369

4. Niller HH, Wolf H, Minarovits J: Regulation and dysregulation of Epstein-Barr virus latency: implications for the development of autoimmune disease. Autoimmunity 2008:41(4):298-328

5. Studahl M, Hagberg L, Rekvdar E, Bergstrom T: Herpesvirus DNA detection in cerebrospinal fluid: difference in clinical presentation between alph-, beta-, and gamma-herpes viruses. Scand J Infect Dis 2000;32(3):237-248

6. Lau AH, Soltys K, Sindhi RK, et al: Chronic high Epstein-Barr viral load carriage in pediatric small bowel transplant recipients. Pediatr Transplant 2010;14(4):549-553 Epub: Jan 20

Method Name

Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization

Specimen Type

Varies


Necessary Information


Specimen source is required.



Specimen Required


Supplies: Aliquot Tube, 5 mL (T465)

Specimen Type: Fluid

Sources: Spinal fluid, sterile body fluids (peritoneal fluid/ascites, pericardial fluid, pleural fluid/thoracentesis, amniotic, or ocular

Preferred: Sterile screw-cap 5-mL aliquot tube

Acceptable: Sterile container

Specimen Volume: 0.5 mL

Collection Instructions: Do not centrifuge.

 

Supplies: Aliquot Tube, 5 mL (T465)

Specimen Type: Fluid

Sources: Respiratory; bronchial washing, bronchoalveolar lavage, nasopharyngeal aspirate or washing, sputum, or tracheal aspirate

Container/Tube:

Preferred: Sterile screw-cap 5-mL aliquot tube

Acceptable: Sterile container

Specimen Volume: 1.5 mL

 

Supplies:

Culturette (BBL Culture Swab) (T092)

M4-RT (T605)

Specimen Type: Swab

Sources: Eye swabs and upper respiratory swabs (nasal, throat)

Container/Tube: Multimicrobe media (M4-RT) and Eswabs

Collection Instructions: Place swab back into multimicrobe media (M4-RT, M4 or M5)

 

Specimen Type: Bone marrow

Container/Tube: Lavender top (EDTA) only

Specimen Volume: 0.5 mL

Additional Information: Clotted specimens will be rejected.

 

Supplies: M4-RT (T605)

Specimen Type: Tissue

Sources: Brain, colon, kidney, liver, lung, etc.

Preferred: Multimicrobe medium (M4-RT)

Acceptable: Sterile container containing 1 mL to 2 mL of sterile saline or multimicrobe medium (M4-RT, M4 or M5)

Specimen Volume: Entire collection

Collection Instructions: Submit only fresh tissue.


Specimen Minimum Volume

Body Fluid, Ocular Fluid, Spinal Fluid: 0.3 mL
Respiratory Specimens: 1 mL
Tissue: 2 × 2-mm biopsy

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) 7 days
  Frozen  7 days

Reference Values

Negative

Test Classification

This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

87798

LOINC Code Information

Test ID Test Order Name Order LOINC Value
LEBV Epstein-Barr Virus PCR 23858-4

 

Result ID Test Result Name Result LOINC Value
SRC67 Specimen Source 31208-2
81239 Epstein-Barr Virus PCR 23858-4

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.

Mayo Clinic Laboratories | Neurology Catalog Additional Information:

mml-CNS-Infections, mml-Pediatric, mml-Spinal-Corde